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Cell Line Name：

Host Cell:

Ba/F3

Application:

Anti-proliferation assay and PD assay

Freeze Medium:

90% FBS+10% DMSO

Complete Culture Medium:

RPMI-1640+10%FBS

Mycoplasma Status:

Negative

CSF3R T618I lies within the extracellular domain proximal to the transmembrane domain of the Csf3r protein (PMID: 23656643). T618I confers a gain of function to the Csf3r protein as demonstrated by increased proliferation and increased downstream Jak-Stat signaling in transformed cells in culture (PMID: 23656643).

1.WB of  CSF3R T618I/BaF3

2. Anti-proliferation assay

Figure 2. Anti-proliferation assay of three reference compounds on the CSF3R T618I/BaF3.