,,

產品搜索

聯系人：蔣經理
電話：4008750250
號碼：
手機：18066071954
地址：南京市棲霞區緯地路9號
Email： zhangxiangwen@cobioer.com

名稱	BCR-ABL1 Y253H/T315I/BaF3
型號	CBP73263
報價
特點	BCR-ABL1 [Y253H/T315I]/BaF3,母細胞：BaF3，凍存條件：90% FBS+10% DMSO

BCR-ABL1 Y253H/T315I/BaF3

CBP73263
I. Introduction
Cell Line Name：	BCR-ABL1 [Y253H/T315I]/BaF3
Host Cell:	Ba/F3
Stability:	16 passages (in-house test, that not means the cell line will be instable beyond the passages we tested.)
Application:	Anti-proliferation assay and PD assay
Freeze Medium:	90% FBS+10% DMSO
Complete Culture Medium:	RPMI-1640+10%FBS
Mycoplasma Status:	Negative

II.Background
Presence of a BCR-ABL1 fusion gene is necessary for the pathogenesis of CML. In up to 95% of cases, a t(9;22) (q34;q11) translocation results in the BCR-ABL1 fusion gene (Faderl et al. 1999). This translocation results in the Philadephia chromosome. In rare CML cases lacking the traditional t(9;22) translocation, other translocations result in the creation of the BCR-ABL1 fusion gene, which sometimes involve multiple chromosomes. ABL1 is a tyrosine kinase, and, in normal cells, it plays a role in cellular differentiation and regulation of the cell cycle. The BCR-ABL1 fusion gene creates a constitutively active tyrosine kinase, which leads to uncontrolled proliferation.

III. Representative Data
1. WB of BCR-ABL1 [Y253H/T315I]/BaF3
2.Sanger of BCR-ABL1 [Y253H/T315I]/BaF3 Figure 2. BCR-ABL1 Y253H Figure 3. BCR-ABL1 T315I Figure 4. BCR ABL1 Breakpoint

3. Anti-proliferation assay

Figure 5. CTG Proliferation Assay of BaF3 BCR-ABL1 T315I Y253H (C2).