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Cell Line Name：

Host Cell:

Ba/F3

Application:

Anti-proliferation assay and PD assay

Freeze Medium:

90% FBS+10% DMSO

Complete Culture Medium:

RPMI-1640+10%FBS

Mycoplasma Status:

Negative

Human cKit with D816V substitution, GenBank Accession No. NM_000222, a.a. 544-end with N-terminal His- tag, expressed in a Baculovirus infected Sf9 cell expression system. MW=53 kDa.

1.WB of  c-Kit D816V/BaF3

2. Sanger of c-Kit D816V/BaF3

3. Anti-proliferation assay

Figure 3 Anti-proliferation assay of three reference compounds on the c-Kit D816V/BaF3.